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Add NanoSparkTM EVEN-T Soluble T Cell Activator to your cart now!
Part Number: EVEN-T
$300.00
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The NanoSparkTM EVEN-T Soluble T Cell Activator is a culture-ready reagent for in vitro activation and expansion of human T cells. The nanoscale of the NanoSparkTM EVEN-T protein complex allows for sterile filtration and easy removal of the activating reagent via standard media exchange or simple centrifugal cell washing. The percentage of CD4+ T cells can be increased by adding increasing amounts of EVEN-T to the culture media, effectively modulating the CD4+:CD8+ ratio during the expansion step. The functional dose range is media-specific and can be tuned to your platform needs.
ConvenientSoluble, magnetic bead-free, protein-based, and ready-to-use.
High PerformanceProprietary CD4+:CD8+ shifting technology with excellent total T cell expansion.
ReliableEmploys anti-CD3/CD28 antibodies and a proprietary additive to modulate the CD4+:CD8+ ratio in a dose-dependent manner.
A T cell activator is a substance or molecule that stimulates the activation and proliferation of T cells, enabling the uptake of genetic constructs for CAR-T applications and encouraging rapid and robust expansion in in vitro culture.
In the body, CD8+ and CD4+ T cells require antigen-presenting cells (APCs) to initiate activation. This is a complex and costly process that is impractical to replicate ex vivo. Simplified reagent-based activation and expansion protocols using T cell activators are largely preferred.
Reagent-based activation is also ideally suited for CAR-T therapy development. This is especially true when large quantities are required for T cell immunotherapy research and manufacturing.
The NanoSparkTM EVEN-T Soluble T Cell Activator contains self-assembling protein nanoparticles with functional antibodies bound to the surface through high-affinity non-covalent interactions. These anti-CD3 and anti-CD28 antibodies are arranged in a proprietary biophysical combination on the NanoSparkTM platform. EVEN-T is formulated with a safe, proprietary additive that enables shifting of the CD4+:CD8+ T cell ratio throughout the expansion.
The NanoSparkTM EVEN-T Soluble T Cell Activator activates resting human CD3+ T cells originating from purified T lymphocytes or patient peripheral blood mononuclear cells (PBMCs).
NanoSpark(TM) EVEN-T is first in class – there are no other T cell activator products that allow researchers to control the CD4+:CD8+ T cell ratio during expansion. Competing products introduce clinical risks to the process by using synthetic ingredients that may contaminate T cell therapy if not painstakingly removed from the expanded culture.
The NanoSparkTM product line is modular and plug-and-play by design, using a proprietary self-assembly protein nanoparticle complex that is inherently soluble and can be filter sterilized.
Inquire About Nanotein’s T Cell Activator
Need help getting started? Want to benefit most from modulating the CD4+:CD8+ T cell ratio in your immunotherapy research? Contact the Nanotein team today to learn more.
| Product Line | NanoSparkTM |
| Cell Type | T cells |
| Sample Type | PBMC, cell culture, T cell isolate |
| Reactivity | Human |
| Application | T cell activators, T cell manufacturing, immunotherapy |
| Grade | Research grade |
| Quantity | 1 mL |
| Contents | Activator contents suspended in phosphate-buffered saline glycerol |
| Functional Testing | ≥80% expected proliferation of T cells on day 7 |
| Sterility | Sterile 0.2 µm filtered post-aseptic production |
| Mycoplasma | Negative |
| Storage | Keep frozen at -80°C for long-term storage Once thawed, store at 4°C protected from light for up to one month |
| Shipping Condition | Frozen on dry ice |
| Product Weight | 20 g (0.044 lbs) |
| Product Dimensions | 13 x 13 x 38 mm (0.5 x 0.5 x 1.5 in) |
The NanoSparkTM EVEN-T Soluble T Cell Activator is used in vitro to induce activation, modulate CD4+/CD8+ T cell ratio, and CAR gene delivery uptake T cells. It is intended for ex vivo activation and expansion of CD3+ T Lymphocytes (human resting T cells) from T cell isolates or peripheral blood mononuclear cells (PBMCs). The activator should be stored at -80 °C long-term. Once thawed, store at 4 °C protected from light for up to one month.
The NanoSparkTM EVEN-T Soluble T Cell Activator is designed for use with a cytokine-supplemented T cell expansion medium. Suggested protocols have been developed for common cytokine-supplemented strategies. Optimization of the provided general protocol information may be necessary depending on your experimental objectives.
Interleukin 2 (IL-2) is a potent general T cell growth factor essential for the long-term proliferation of activated T cells.
IL-2 should be added to culture media at 20 ng/mL final concentration. Check back for detailed EVEN-T IL-2 protocol, but see analogous IL-2 STEM-T Protocol and Data Sheet for general guidelines.
Interleukin 7 (IL-7) is a cytokine required in early T cell development that promotes T cell survival by up-regulating the expression of anti-apoptotic genes. Interleukin 15 (IL-15) is an inflammatory cytokine that supports the homeostasis of naive CD8+ T cells. Combining IL-7 and IL-15 in culture promotes the enrichment of self-renewing T cells like TSCM.
IL-7 and IL-15 should be added to culture media at 10 ng/mL final concentration of each. For detailed information, please refer to the IL-7/IL-15 EVEN-T Protocol and Data Sheet.
EVEN-T has yet to be optimized in Wilson Wolf Sterile GRex6M cell culture vessels, so check back for a detailed protocol. Until then, please see IL-7/IL-15 GRex6M STEM-T Protocol and Data Sheet for general guidelines.
If using transduction (optional), wait 24-72 hours after adding NanoSparkTM EVEN-T Soluble T Cell Activator to the cell culture before adding the viral vector. If using electroporation, then electroporate 72 hours after activator addition.
These protocols are recommended and include suggested catalog numbers of cytokine supplements and media to use.
View EVEN-T Cell Data Sheet 2023 (IL-7 & IL-15)
Lot-specific EVEN-T CoA will be shipped with the product. Contact customer service if additional copies are required.
View Nanotein Example STEM-T CoA
Carefully read product SDS before use.
View NanoSparkTM EVEN-T SDS
The underlying NanoSparkTM platform has undergone biocompatibility testing when formulated with antibodies specific to our T cell activator, STEM-T, which we consider a significant derisking step for the platform as a whole. The details are as follows:
The composition of the NanoSparkTM reagent is protein-based, and consequently naturally biodegradable, which provides a significant safety advantage over magnetic bead-based and synthetic products. A reputable medical device CRO was commissioned to complete the in vitro & in vivo ISO 10993 biocompatibility testing. The tests were run at >20X the predicted possible carry-over amount, which resulted in no toxicity or adverse effects:
| ISO 10993
Biocompatibility Testing |
Test | Type of Test | Result | Product Tested |
| ISO 10993-5 | Cytotoxicity | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-10 | Sensitization | In vivo: Guinea Pig | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-10 | Irritation/Intracutaneous Reactivity | In vivo: Rabbits | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-11 | Acute Systemic Toxicity | In vivo: Mice | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-11 | Pyrogenicity | In vivo: Rabbits | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-4 | Hemocompatibility | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-3 | Genotoxicity Ames | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-3 | Genotoxicity Mouse Lymphoma Forward Mutation | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
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