NanoSpark™ STEM-T Soluble T Cell Activator

A: Yes, in ~Q4 of 2023. Please contact us for a more detailed updated timeline.

A: Yes it works with both.

A: Yes, our team is here to help you get the best performance from our product as possible! Please contact us at our contact page.

A: The NanoSpark™ platform product line contains self-assembling protein nanoparticles with functional antibodies bound to the surface through high-affinity non-covalent interactions. These antibodies are specific to the cell type being activated and expanded ex vivo. The NanoSpark™ STEM-T Soluble T Cell Activator is intended for use with resting human CD3+ T cells. These T cells may originate from purified T lymphocytes or patient peripheral blood mononuclear cells (PBMCs). NanoSpark™ STEM-T uses anti-CD3 and anti-CD28 antibodies in a proprietary biophysical combination that leads to primary and costimulatory signals that favor the production of stem-like CD3+ T cell populations. 

A: Fresh or thawed (from cryopreserved) CD3+ T lymphocytes or peripheral blood mononuclear cells (PBMCs) may be used. The NanoSpark™ STEM-T Soluble T Cell Activator is designed to be paired with a T-cell expansion medium supplemented with recombinant human IL-2 or IL-7/IL-15 for cell culture. Applicable data sheets may be found here for use with IL-2  or IL-7/IL-15, including best practices. 

A: Typical T-cell activation and expansion requires one to two weeks of cell culture maintenance to obtain sufficient numbers of expanded cells. However, this is dependent on the downstream application. Recent work has shown shorter expansion times (three to five days) lead to the preservation of stemness in cultured T cells. We encourage you to research and validate your application-specific protocol internally. Please contact us for assistance.

A: The NanoSpark™ product line is modular and plug-and-play by design. Our technology harnesses a self-assembling protein nanoparticle complex that displays functional antibodies—it’s inherently soluble and can be filter-sterilized. 

Competitor platforms are not modular. They contain synthetic beads that cannot go through filter sterilization or are polysaccharide-based and can over stimulate the cells. 

In addition, our NanoSpark™ STEM-T Soluble T Cell Activator consistently produces a greater number of CD8+ T cells with uniquely maintained stem-like phenotypes. Retaining less-differentiated stemness of T cells directly correlates with improved long-term self-renewal in vivo and increased positive clinical outcomes.