NanoSpark STEM-T Soluble T Cell Activator

Description

Product Highlights

The NanoSpark STEM-T Soluble T Cell Activator is a culture-ready reagent for in vitro activation and expansion of human T cells. The nanoscale of the NanoSpark STEM-T protein complex allows for sterile filtration and removal of excess reagent via either media exchange or simple centrifugal cell washing.

Convenient
Soluble, magnetic bead-free, protein-based, and ready-to-use.
High Performance
Excellent total T cell expansion with industry-leading clinically desirable CD8+ stem cell memory T cell expansion.
Reliable
Employs anti-CD3/CD28 antibodies and maintains high cell viability.

Additional information

Weight	7 lbs
Dimensions	9 × 9 × 9 in

Documents

Data Sheets

Certificate of Analysis (CoA) Example

Safety Data Sheet (SDS)

FAQ's

Q: Will there be a GMP version of this available?

Q: Does the NanoSpark activator work for T cell expansion from PBMCs and purified T cell populations?

Q: Do you provide application/troubleshooting support?

Quality & Safety

Nanotein assessed the NanoSpark activator with adherence to rigorous ISO 10993 standards in preparation for GMP manufacturing. The composition of the NanoSpark reagent is protein-based, and consequently naturally biodegradable, which provides a significant safety advantage over magnetic bead-based and synthetic products. A reputable medical device CRO was commissioned to complete the in vitro & in vivo ISO 10993 biocompatibility testing. The tests were run at >20X the predicted possible carry-over amount, which resulted in no toxicity or adverse effects:

ISO 10993 Biocompatibility Testing	Test	Type of Test	Result	Product Tested
ISO 10993-5	Cytotoxicity	In vitro	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-10	Sensitization	In vivo: Guinea Pig	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-10	Irritation/Intracutaneous Reactivity	In vivo: Rabbits	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-11	Acute Systemic Toxicity	In vivo: Mice	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-11	Pyrogenicity	In vivo: Rabbits	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-4	Hemocompatibility	In vitro	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-3	Genotoxicity Ames	In vitro	No Toxicity/Adverse Effect	NanoSpark STEM-T
ISO 10993-3	Genotoxicity Mouse Lymphoma Forward Mutation	In vitro	No Toxicity/Adverse Effect	NanoSpark STEM-T

NanoSpark STEM-T Soluble T Cell Activator

Description

Product Highlights

Convenient

High Performance

Reliable