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Add NanoSparkTM STEM-T Soluble T Cell Activator to your cart now!
$275.00
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The NanoSparkTM STEM-T Soluble T Cell Activator is a culture-ready reagent for in vitro activation and expansion of human T cells. The nanoscale of the NanoSparkTM STEM-T protein complex allows for sterile filtration and removal of excess reagent via either media exchange or simple centrifugal cell washing.
ConvenientSoluble, magnetic bead-free, protein-based, and ready-to-use.
High PerformanceExcellent total T cell expansion with industry-leading clinically desirable CD8+ stem cell memory T cell expansion.
ReliableEmploys anti-CD3/CD28 antibodies and maintains high cell viability.
| Product Line | NanoSparkTM |
| Cell Type | T cells |
| Sample Type | PBMC, cell culture, T cell isolate |
| Reactivity | Human |
| Application | T cell activators, T cell manufacturing, immunotherapy |
| Grade | Research grade |
| Quantity | 1 mL |
| Contents | Activator contents suspended in phosphate-buffered saline glycerol |
| Functional Testing | ≥80% expected proliferation of T cells on day 7 |
| Sterility | Sterile 0.2 µm filtered post-aseptic production |
| Mycoplasma | Negative |
| Storage | Keep frozen at -80°C for long-term storage Once thawed, store at 4°C protected from light for up to one month |
| Shipping Condition | Frozen on dry ice |
| Product Weight | 20 g (0.044 lbs) |
| Product Dimensions | 13 x 13 x 38 mm (0.5 x 0.5 x 1.5 in) |
The NanoSparkTM STEM-T Soluble T Cell Activator is used in vitro to induce activation and CAR gene delivery uptake in stemlike CD8+ cells via anti-CD3/CD28 pathways. It is intended for ex vivo activation and expansion of CD3+ T Lymphocytes (human resting T cells) from T cell isolates or peripheral blood mononuclear cells (PBMCs). The activator should be stored at -80 °C long-term. Once thawed, store at 4 °C protected from light for up to one month.
The NanoSparkTM STEM-T Soluble T Cell Activator is designed for use with a cytokine-supplemented T cell expansion medium. Suggested protocols have been developed for common cytokine-supplemented strategies. Optimization of the provided general protocol information may be necessary depending on your experimental objectives.
Interleukin 2 (IL-2) is a potent general T cell growth factor essential for the long-term proliferation of activated T cells.
IL-2 should be added to culture media at 20 ng/mL final concentration. For detailed information, please refer to the IL-2 STEM-T Protocol and Data Sheet.
Interleukin 7 (IL-7) is a cytokine required in early T cell development that promotes T cell survival by up-regulating the expression of anti-apoptotic genes. Interleukin 15 (IL-15) is an inflammatory cytokine that supports the homeostasis of naive CD8+ T cells. Combining IL-7 and IL-15 in culture promotes the enrichment of self-renewing T cells like TSCM.
IL-7 and IL-15 should be added to culture media at 10 ng/mL final concentration of each. For detailed information, please refer to the IL-7/IL-15 STEM-T Protocol and Data Sheet.
For use in Wilson Wolf Sterile GRex6M cell culture vessels, please see IL-7/IL-15 GRex6M STEM-T Protocol and Data Sheet for details.
If using transduction (optional), wait 24-72 hours after adding NanoSparkTM STEM-T Soluble T Cell Activator to the cell culture before adding the viral vector. If using electroporation, then electroporate 72 hours after activator addition.
Nanotein assessed the NanoSparkTM activator with adherence to rigorous ISO 10993 standards in preparation for GMP manufacturing. The composition of the NanoSparkTM reagent is protein-based, and consequently naturally biodegradable, which provides a significant safety advantage over magnetic bead-based and synthetic products. A reputable medical device CRO was commissioned to complete the in vitro & in vivo ISO 10993 biocompatibility testing. The tests were run at >20X the predicted possible carry-over amount, which resulted in no toxicity or adverse effects:
| ISO 10993 Biocompatibility Testing | Test | Type of Test | Result | Product Tested |
| ISO 10993-5 | Cytotoxicity | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-10 | Sensitization | In vivo: Guinea Pig | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-10 | Irritation/Intracutaneous Reactivity | In vivo: Rabbits | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-11 | Acute Systemic Toxicity | In vivo: Mice | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-11 | Pyrogenicity | In vivo: Rabbits | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-4 | Hemocompatibility | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-3 | Genotoxicity Ames | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
| ISO 10993-3 | Genotoxicity Mouse Lymphoma Forward Mutation | In vitro | No Toxicity/Adverse Effect | NanoSpark STEM-T |
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