Description
The NanoSparkTM GROW-NK Soluble Activator is a culture-ready, feeder-free reagent for in vitro activation and expansion of human NK cells. The nanoscale of the NanoSparkTM GROW-NK protein complex allows for sterile filtration and removal of excess reagent via either media exchange or simple centrifugal cell washing.
Quality & Safety
The underlying NanoSparkTM platform has undergone biocompatibility testing when formulated with antibodies specific to our T cell activator, STEM-T, which we consider a significant derisking step for the platform as a whole. The details are as follows:
The composition of the NanoSparkTM reagent is protein-based, and consequently naturally biodegradable, which provides a significant safety advantage over magnetic bead-based and synthetic products. A reputable medical device CRO was commissioned to complete the in vitro & in vivo ISO 10993 biocompatibility testing. The tests were run at >20X the predicted possible carry-over amount, which resulted in no toxicity or adverse effects:
ISO 10993
Biocompatibility Testing |
Test |
Type of Test |
Result |
Product Tested |
ISO 10993-5 |
Cytotoxicity |
In vitro |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-10 |
Sensitization |
In vivo: Guinea Pig |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-10 |
Irritation/Intracutaneous Reactivity |
In vivo: Rabbits |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-11 |
Acute Systemic Toxicity |
In vivo: Mice |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-11 |
Pyrogenicity |
In vivo: Rabbits |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-4 |
Hemocompatibility |
In vitro |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-3 |
Genotoxicity Ames |
In vitro |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |
ISO 10993-3 |
Genotoxicity Mouse Lymphoma Forward Mutation |
In vitro |
No Toxicity/Adverse Effect |
NanoSpark STEM-T |