Nanotein & Akron Bio Partner for cGMP production of NanoSpark® STEM-T Soluble T Cell Activator. Read More

NanoSpark™ STEM-T
Soluble T Cell Activator – 1 mL

The NanoSparkTM STEM-T Soluble T Cell Activator reagent kit is an easy-to-use T cell activator for T cell expansion, including CAR-T cells.
  • Soluble format
  • All protein-based
  • No magnetic components
  • High total & stemlike CD8+ T cell production
  • Anti-CD3/CD28 based
  • GMP in production, available ~Q4 2024

Add NanoSparkTM STEM-T Soluble T Cell Activator to your cart now!

Part Number: STEM-T

$275.00Read more

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Description

The NanoSparkTM STEM-T Soluble T Cell Activator is a culture-ready reagent for in vitro activation and expansion of human T cells. The nanoscale of the NanoSparkTM STEM-T protein complex allows for sterile filtration and removal of excess reagent via either media exchange or simple centrifugal cell washing.

NanoSparkTM STEM-T: High Stemlike T cell Production

NanoSparkTM STEM-T is best in class, particularly for preserving stemlike CD8+ T cells important for higher efficacy. Competing products introduce clinical risks to the process by using synthetic ingredients that may contaminate T cell therapy if not painstakingly removed from the expanded culture.

The NanoSparkTM product line is modular and plug-and-play by design, using a proprietary self-assembly protein nanoparticle complex that is inherently soluble and can be filter sterilized.

How Does the NanoSparkTM STEM-T Soluble T Cell Activator Work?

The NanoSparkTM STEM-T Soluble T Cell Activator contains self-assembling protein nanoparticles with functional antibodies bound to the surface through high-affinity non-covalent interactions. These anti-CD3 and anti-CD28 antibodies are arranged in a proprietary biophysical combination on the NanoSparkTM platform to drive best-in-class performance that favors stemlike CD8+ T cell production.

The NanoSparkTM STEM-T Soluble T Cell Activator activates resting human CD3+ T cells originating from purified T lymphocytes or patient peripheral blood mononuclear cells (PBMCs).

Inquire About Nanotein’s T Cell Activator

Need help getting started? Want to benefit most from your T cell activation and expansion? Contact the Nanotein team today to learn more.


  • Convenient

    Soluble, magnetic bead-free, protein-based, and ready-to-use.


  • High Performance

    Excellent total T cell expansion with industry-leading clinically desirable CD8+ stem cell memory T cell expansion.


  • Reliable

    Employs anti-CD3/CD28 antibodies and maintains high cell viability.

Product Details

Product Line NanoSparkTM
Cell Type T cells
Sample Type PBMC, cell culture, T cell isolate
Reactivity Human
Application T cell activators, T cell manufacturing, immunotherapy
Grade Research grade
Quantity 1 mL
Contents Activator contents suspended in phosphate-buffered saline glycerol
Functional Testing ≥80% expected proliferation of T cells on day 7
Sterility Sterile 0.2 µm filtered post-aseptic production
Mycoplasma Negative
Storage Keep frozen at -80°C for long-term storage
Once thawed, store at 4°C protected from light for up to one month
Shipping Condition Frozen on dry ice
Product Weight 20 g (0.044 lbs)
Product Dimensions 13 x 13 x 38 mm (0.5 x 0.5 x 1.5 in)

Documentation & Protocols

Consult the data sheets and supporting documentation for usage instructions and additional details, or explore the recommended protocols, which include suggested catalog numbers for cytokine supplements and media.

Data Sheets

These protocols are recommended and include suggested catalog numbers of cytokine supplements and media to use.

The NanoSparkTM STEM-T Soluble T Cell Activator is used in vitro to induce activation and CAR gene delivery uptake in stemlike CD8+ cells via anti-CD3/CD28 pathways. It is intended for ex vivo activation and expansion of CD3+ T Lymphocytes (human resting T cells) from T cell isolates or peripheral blood mononuclear cells (PBMCs). The activator should be stored at -80 °C long-term. Once thawed, store at 4 °C protected from light for up to one month.

The NanoSparkTM STEM-T Soluble T Cell Activator is designed for use with a cytokine-supplemented T cell expansion medium. Suggested protocols have been developed for common cytokine-supplemented strategies. Optimization of the provided general protocol information may be necessary depending on your experimental objectives.

STEM-T Expansion Protocol & Data Sheet

Certificate of Analysis (CoA)

Lot-specific STEM-T CoA will be shipped with the product. Contact customer service if additional copies are required.

Nanotein Example STEM-T CoA

Safety Data Sheet (SDS)

Carefully read product SDS before use.

NanosparkTM STEM-T SDS

Tips for Best Results Using the NanoSparkTM STEM-T Soluble T Cell Activator

  • Seed CD3+ T cells/PBMCs at 7 x 105 cells/cm2 in culture media.
  • Add volume of NanoSparkTM STEM-T Soluble T Cell Activator as recommended in table above to achieve specific goals.
  • Incubate cells at 37 °C and 5% CO2 in a humidified incubator.
  • For cryopreserved cells, gently thaw cells and exchange them into culture media before starting.
  • For optimal cell expansion and maintenance, ensure the activator is in culture media (conditioned or fresh) for at least 72 hours (removal is unnecessary for expansion).
  • Be sure to add fresh cytokine-supplemented culture media every two to three days as needed.

If using transduction (optional), wait 24-72 hours after adding NanoSparkTM STEM-T Soluble T Cell Activator to the cell culture before adding the viral vector. If using electroporation, then electroporate 72 hours after activator addition.

Data & Research

Enhanced T Cell Expansion and Viability with NanoSparkTM STEM-T Soluble T-Cell Activator

A) Fold Change of CD3+ Cells, B) % of CD4+ & CD8+ Cells, C) CD4+ & CD8+ T-Cell Phenotype Percentage. NanoSparkTM STEM-T Soluble T-Cell Activator was cultured in StemCell’s ImmunoCult-XF T Cell Expansion Medium (xeno-free) supplemented with IL-7 and IL-15. Cells were expanded for 10 days and analyzed on a flow cytometer on days 0, 7, and 10. Cells were labeled with CD4+, CD8+, CD45RA, CCR7, and CD95 fluorescent antibodies (Schmueck-Henneresse et al 2017). Expansion with NanoSparkTM STEM-T Soluble T-Cell Activator enhances total cell viability and stimulates expansion of CD8+ T-Cells and promotes the stem-like phenotypes of CD4+ and CD8+ T-Cells: Tnaive, Tscm, and Tcm.

Optimized T-Cell Expansion and Phenotypic Development with NanoSparkTM STEM-T in G-Rex6M System

A) Fold Change of CD3+ Cells, B) % of CD4+ & CD8+ Cells, C) CD4+ & CD8+ T-Cell Phenotype Percentage. NanoSparkTM STEM-T Soluble T-Cell Activator was cultured in StemCell’s ImmunoCult-XF T Cell Expansion Medium (xeno-free) supplemented with IL-7 and IL-15. Cells were expanded for 10 days in a G-rex6M and analyzed on a flow cytometer on days 0, and 10. Cells were labeled with CD4+, CD8+, CD45RA, CCR7, and CD95 fluorescent antibodies (Schmueck-Henneresse et al 2017). Expansion with NanoSparkTM STEM-T Soluble T-Cell Activator enhances total cell viability and stimulates expansion of CD8+ T-Cells and promotes the stem-like phenotypes of CD4+ and CD8+ T-Cells: Tnaive, Tscm, and Tcm.

FAQs

Quality & Safety

Nanotein assessed the NanoSparkTM platform with adherence to rigorous ISO 10993 standards in preparation for GMP manufacturing. The composition of the NanoSparkTM reagent is protein-based, and consequently naturally biodegradable, which provides a significant safety advantage over magnetic bead-based and synthetic products. A reputable medical device CRO was commissioned to complete the in vitro & in vivo ISO 10993 biocompatibility testing.  The tests were run at >20X the predicted possible carry-over amount, which resulted in no toxicity or adverse effects:

ISO 10993  Biocompatibility Testing Test Type of Test Result Product Tested
ISO 10993-5 Cytotoxicity In vitro No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-10 Sensitization In vivo: Guinea Pig No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-10 Irritation/Intracutaneous Reactivity In vivo: Rabbits No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-11 Acute Systemic Toxicity In vivo: Mice No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-11 Pyrogenicity In vivo: Rabbits No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-4 Hemocompatibility In vitro No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-3 Genotoxicity Ames In vitro No Toxicity/Adverse Effect NanoSpark STEM-T
ISO 10993-3 Genotoxicity Mouse Lymphoma Forward Mutation In vitro No Toxicity/Adverse Effect NanoSpark STEM-T

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